Comparison of some minor activities accompanying a preparation of sodium-plus-potassium ion-stimulated adenosine triphosphatase from pig brain
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The effects of long chain fatty acids on sodium plus potassium ion-stimulated adenosine triphosphatase of rat brain.
Long chain fatty acids inhibit the Mg2+-dependent Na+ + K+-stimulated ATPase of rat brain microsomal membrane preparations without any significant effect on the “basic” Mg*+-stimulated component. The magnitude of inhibition increases with the chain length, reaching a maximum with myristate followed by a decline with higher fatty acids. Myristate (5 X 10e5 M) produces about 45 % inhibition of th...
متن کاملBinding of adenosine triphosphate to sodium and potassium ion-stimulated adenosine triphosphatase.
In order to characterize a step in the reaction sequence of sodium and potassium ion-dependent adenosine triphosphatase (EC 3.6.1.3), the binding of radioactive adenosine triphosphate to kidney cell membranes was estimated by flow dialysis in the presence of a chelator of divalent cations, which prevented hydrolysis of the adenosine triphosphate. Adenosine triphosphate was bound to a single sit...
متن کاملDistribution of sodium-plus-potassium-stimulated adenosine-triphosphatase activity in isolated nerve-ending particles.
1. A rapid method for the isolation of nerve-ending particles from brain is described. This involved the centrifugation of the large-granule fraction over a discontinuous density gradient consisting of 3% (w/v) and 13% (w/v) Ficoll dissolved in 0.32m-sucrose. The results of the biochemical as well as morphological identification of nerve-ending particles are given. 2. Approx. 20% of the (Na(+)+...
متن کاملPhysiological consequences of the cellular distribution of sodium-plus-potassium ion-dependent adenosine triphosphatase.
Evers, C., Murer, H. & Kinne, R. (1978) Biochem. J. 172,49-56 Hoffman, N., Thees, M. & Kinne, R. (1976) P#i&ers Arch. 262,147-156 Kinne, R. (1976) MTP Znt. Rev. Sci: Kidney and Urinary Tract Physiology (Thurau, K., ed.), vol. 2, pp. 169-210, University Park Press, Baltimore Kinne, R. & Faust, G. (1977) Biochem. J. 168,311-314 Kinne, R., Shlatz. L. J., Kinne-Saffran, E. & Schwartz, J. L. (1975) ...
متن کاملPhosphorylation by inorganic phosphate of sodium plus potassium ion transport adenosine triphosphatase. Four reactive states.
Native solium and potassium adenosine triphosphatase from guinea pig kidney accepted a phosphate group from radioactive inorganic phosphate to form an acyl phosphate bond at the active site in the presence or absence of sodium ion. Magnesium ion was always required. In the presence of sodium ion and absence of adenosine triphosphate, there was no phosphorylation by inorganic phosphate. Addition...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1968
ISSN: 0306-3283
DOI: 10.1042/bj1060113